The Algavalue project intends to optimize the biotechnological process of squalene production, of high health interest given its anti-oxidative and anti-carcinogenic properties, from a heterotrophic microalgae that, in certain growing conditions, besides producing significant amounts of squalene, produces other compounds of high value and commercial interest, such as docosahexaenoic acid (DHA), carotenoids and others. In addition, the project intends to develop strategies for the bio-refineries by-products and waste valorization, promoting more environmentally sustainable practices and adding commercial value to this activity.